IHC staining of tumors from mice coinjected with CAFs/GBC-SD and a semiquantitative analysis revealed a strikingly higher expression of Ki-67, EMT and cancer stemness markers in tumor cells than was observed in subcutaneous tumors from mice injected with GBC-SD cells alone (Fig.?3d-e). of stemness markers (CD44, Nanog, Oct4 and Sox2), and epithelial-mesenchymal transition markers (E-cadherin and vimentin) at protein level were analyzed and plotted. n?=?three independent experiments, *P?P?P?P?P?P?P?P?P??150?m was plotted and calculated. HLI-98C The size pub represents 50?m. Magnification can be ?200, and HLI-98C size bars?=?50?m. n?=?three independent tests, **P?P?P?HLI-98C paracrine of TSP-4 signaling for the proliferation of GBC cells. (A-C) NOZ and GBC-SD cells had been incubated with rh-TSP-4, rh-TSP-4?+?anti-2 or anti-2, the proliferation of GBC cells was dependant on MTT after that, Colony formation and Edu assay respectively. n?=?three independent tests, *P?P?P?P?P?P?P?P? SCKL (B-D) GBC-SD and NOZ cells had been treated with rh-TSP-4, si-HSF1or rh-TSP-4?+?si-HSF1, then your proliferation of GBC cells was dependant on MTT, Colony formation and Edu assay respectively. n?=?three independent tests, *P?P?P?P?P?